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KMID : 0358320090500010081
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Korean Journal of Urology
2009 Volume.50 No. 1 p.81 ~ p.88
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Effect of Decursin on the Expression of ?-Catenin and Matrix Metalloproteinase-7 in Prostate Cancer Cell Lines
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Choi Ik-Joon
Min Kweon-Sik
Kang Dong-Il
Song Gyu-Yong
Oh Sang-Taek
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Abstract
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Purpose: Alterations in the Wnt/?-catenin pathway are associated with the development and progression of human prostate cancer. Decursin can attenuate the Wnt/?-catenin pathway. We investigated the relationship between the Wnt/?-catenin pathway and decursin in prostate cancer cells.
Materials and Methods: PC-3 and LNCaP cell lines were used. Cell viability was measured with methyl-thiazole tetrazolium bromide(MTT) assays, and cell apoptosis analysis was performed by FACScan. The amount of ?-catenin protein after treatment with decursin was measured by Western blot analysis. Expression of MMP-7 mRNA was detected by real-time polymerase chain reaction(RT-PCR).
Results: Death and apoptosis were increased after treatment with decursin 0.5-100?M in PC-3 and LNCaP cells. This was revealed dose and time-dependent increase of cancer cell death on 24, 48 and 72 hours. FACScan showed an increment of apoptosis on 24, 48 hours. Expression of intracellular ?-catenin protein was decreased dose-dependently in both of prostate cancer cell lines. Decursin reduced MMP-7 mRNA expression on 6, 12, 24, 48 hours dose-dependently.
Conclusions: Decursin affects the viability of prostate cancer cells. Increased cancer cell death was associated with increased apoptosis. This study suggests that decursin may play a role in the treatment of prostate cancer. (Korean J Urol 2009;50:81-88)
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KEYWORD
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Decursin, Beta-catenin, Matrix metalloproteinase, Prostate
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